professional 3 in 1 Electroporation Dermabrasion for scar removal and safe to use

professional 3 in 1 Electroporation Dermabrasion for scar removal and safe to use on many parts of the body including the face area

3 in1 Electroporation

1. Technical parameters.

Power Input: 110V/60Hz or 220V/50Hz

Max. Power: 30 W

Temperature range: 10℃-42℃

2. Accessories

Main unit 1 pc

Electroporation Treatment probe 2 pcs

3M Ultrasonic Treatment Probe 2 pcs

Anode plate 1 pc

Treatment cable 1 pc

Power Cord 1 Pc

Manual 1 pc

Fuse 2 pc

Paper Carton 1 pc

Packing Foam 1 set

3. Functions:

1. Electroporation

2. 3 M Ultrasonic

3. Diamond Dermabrasion

4. Electroporation Working Theory

Electroporation is a mechanical method used to introduce polar molecules into a host cell through the cell membrane. In this procedure, a large electric pulse temporarily disturbs the phospholipid bilayer, allowing molecules like DNA to pass into the cell.

5. Background

Many research techniques in molecular biology require a foreign gene or protein material to be inserted into a host cell. Since the phospholipid bilayer of the plasma membrane has a hydrophobic exterior and a hydrophobic interior (Fig. 1), any polar molecules, including DNA and protein, are unable to freely pass through the membrane.

Figure 1. Diagram of the Phospholipid Bilayer. This image shows the chemical components of the plasma membrane. The polar head groups face outward while the hydrophobic tail groups face inward and interact with one another to hold the membrane together. Polar molecules cannot pass through this membrane without external aid.

Many methods have been developed to surpass this barrier and allow the insertion of DNA and other molecules into the cells to be studied. One such method is electroporation.

The concept of electroporation capitalizes on the relatively weak nature of the phospholipid bilayer's hydrophobic/hydrophilic interactions and its ability to spontaneously reassemble after disturbance. Thus, a quick voltage shock may disrupt areas of the membrane temporarily, allowing polar molecules to pass, but then the membrane may reseal quickly and leave the cell intact.

Procedure

The host cells and the molecules to be inserted into these cells are suspended in solution. The electroporation apparatus is typically commercially produced and purchased, but the basic process inside such an apparatus may be represented in a schematic diagram

Figure 2. Diagram of the basic circuit setup of the electroporation apparatus. This diagram shows the basic electric circuit that provides the voltage for electroporation.
When the first switch is closed, the capacitor charges up and stores a high voltage. When the second switch is closed, this voltage discharges through the liquid of the cell suspension. Typically, 10, 000-100, 000 V/cm (varying with cell size) in a pulse lasting a few microseconds to a millisecond is necessary for electroporation. This electric pulse disturbs the phospholipid bilayer of the membrane and causes the formation of temporary aqueous pores. The electric potential across the membrane of the cell simultaneously rises by about 0.5-1.0 V so that charged molecules (such as DNA) are driven across the membrane through the pores in a manner similar to electrophoresis (Fig 3)

Figure 3. Graphic representation of plasmids containing a foreign DNA insert passing through temporary aqueous pores in the plasma membrane. The actual entry of DNA into the cell cannot be observed with a microscope, but this artist's rendering shows the basic concept of the formation of pores in the membrane through which DNA can pass.

As charged ions and molecules flow through the pores, the cell membrane discharges and the pores quickly close, and the phospholipid bilayer reassembles. The intended molecules should now be inside the cell for further use or study.

6. Advantages and Disadvantages of Electroporation
Several methods other than electroporation are used to transfer polar molecules like DNA into host cells. These other methods include microprecipitates, microinjection, liposomes, and biological vectors. (Melcher, 2000). Electroporation has both advantages and disadvantages compared to these methods.

7. Advantages:
Versatility: Electroporation is effective with nearly all cell and species types.
Efficiency: A large majority of cells take in the target DNA or molecule. In a study on electrotransformation of E. coli, for example, 80% of the cells received the foreign DNA.
Small Scale: The amount of DNA required is smaller than for other methods.
In vivo: The procedure may be performed with intact tissue. A paper published in Developmental Biology showed the successful transfer of a DNA construct with a fluorescent reporter gene into intact mouse brain tissue (Fig 4).

Figure 4. Image of in vivo electroporation in a mouse brain. The mouse brains (telencephalons) in these images are expressing reporter genes (EYFP) introduced in gene constructs by electroporation.

8. Disadvantages:
Cell Damage: If the pulses are of the wrong length or intensity, some pores may become too large or fail to close after membrane discharge causing cell damage or rupture.
Nonspecific Transport: The transport of material into and out of the cell during the time of electropermeability is relatively nonspecific. This may result in an ion imbalance that could later lead to
improper cell function and cell death.

9. Applications
As previously mentioned, electroporation is widely used in many areas of molecular biology research and in the medical field. Some applications of electroporation include:
- DNA Transfection or Transformation: This is likely the most widespread use of electroporation. Specific genes can be cloned into a plasmid and then this plasmid introduced into host cells (bacterial or otherwise) in order to investigate gene and protein structure and function.

Figure 5. Microscope images of the results of transfection by electroporation. In this experiment, a gene construct was inserted by electroporation into the cells shown on the right. The fluorescence of the protein produced by the reporter gene included in this construct shows that the DNA was properly uptaken in the majority of cells. These cells could now be used in further experimentation.

- Direct Transfer of Plasmids Between Cells: Bacterial cells already containing a plasmid may be incubated with another strain that does not contain plasmids but that has some other desireable feature. The voltage of electroporation will create pores, allowing some plasmids to exit one cell and enter another. The desired cells may then be selected by antibiotic resistance or another similar method. This type of transfer may also be performed between species. Thus, large numbers of plasmids may be grown in rapidly multiplying bacterial colonies and then transferred to yeast cells by electroporation for study.

- Induced Cell Fusion: The disruption of the membrane that occurs with the quick pulse of electricity in the electroporation procedure has also been shown to induce fusion of cells.

- Trans-dermal Drug Delivery: Just as electroporation causes temporary pores to form in plasma membranes, studies suggest that similar pores form in lipid bilayers of the stratum corneum- the outermost dead layer of skin. These pores could allow drugs to pass through to the skin to a target tissue. This method of drug delivery would be more pleasant than injection for the patient (not requiring a needle) and could avoid the problems of improper absorption or degradation of oral medication in the digestive system.

- Cancer Tumor Electrochemotherapy: Scientists are investigating the potential of electroporation to increase the effectiveness of chemotherapy. As in electroporation for DNA transfection, the applied electrical pulse would disrupt the membrane of the tumor cell and increase the amount of drug delivered to the site. Some studies have suggested that increased tumor reduction is seen when this method is applied to cancerous cells in animal model systems.

- Gene Therapy: Much like drug delivery, electroporation techniques can allow vectors containing important genes to be transported across the skin and into the target tissue. Once incorporated into the cells of the body, the protein produced from this gene could replace a defective one and thus treat a genetic disorder (Figure 6).

Figure 6. Diagram of the method of gene therapy using electroporation.

10. 3M Ultrasonic
Ultrasonic utilizes sonic vibrations; it works three million times per second (3 MHz). The ultrasonic wave
could stimulate skin tissue and improve permeability of the membrane to promoted collagen tissue, reduced wrinkle and invisible appeared fine line, also lift the skin result for younger and healthy look.
Ultrasonic generate frictional heat which accelerate blood circulation, muscle relaxation, and decompose the cellulite to break down exceed fat.
BC-N2 Ultrasound with 2 probes provided non-surgical lifting. This machine utilizes sonic vibrations to reduce lines, leaving the skin firm and smooth.

11. DIAMONAD Dermabrasion
1. Push in the movable ring of copper nozzle at the hand piece, and pull out the 6x4 sizevacuum hose. Then push in the black plastic ring on the vacuum hose.
2. Put a drop of cream or milk lotion on the vacuum hose nozzle, push in the movable ring and insert in thevacuum hose to the limit.
3. Insert a cotton swab into the Diamond Head, then tight up the Diamond Head on the hand piece. Remove the cotton swab after every treatment.
4. Turn on the power switch.
5. Adjust the vacuum intensity by vacuum Regulator according to the following reference table, the intensity displays on vacuum gauge and check with the client for comfort.
could cause serious injury or death.
6. Store in a dry cool place.

12. Recommended vacuum intensity on treatment area

13. How to Use
1. Plug in the power code into the power socket.
2. Plug in the treatment cable, and connect the treatment probe and Anode plate.
(Note: there are two treatment probes one is big, the other is small, according to the treatment area to choose the suitable treatment probe.)
3. Press the “Power On/off” button to start the machine.
4. Press the “OK” button to select the Ultrasonic function.
5. Press the “Right” button, and then press up and down key to choose the suitable treatment intensity.
6. Press the “Right” button again to choose the Electroporation wave. There are two waves for chosen, One is continuous wave, the other is pulse wave.
7. Press “Start/pause” button to start the function of Electroporation.
8. After treatment, please press “Power On/off” button to switch off the power.

14. How to Operate the 3M Ultrasonic Function
How to Use
1. Plug in the power code into the power socket.
2. Plug in the Ultrasonic treatment cable.
(Note: there are two treatment probes one is big, the other is small, and the big Ultrasonic probe is for face, the small one is for the treatment parts around eyes.)
3. Press the “Power On/off” button to start the machine.
4. Press the button up and down key to choose the suitable treatment time.
5. Press the “Right” button, and then press up and down key to choose the suitable treatment intensity.
6. Press the “Right” button again to choose the ultrasonic wave. There are two waves for chosen, One is continuous wave, the other is pulse wave.
7. Press “Start/pause” button to start the function of Electroporation.
8. After treatment, please press “Power On/off” button to switch off the power.

15. Preparation of Treatment
1. Clean the treatment parts first by clean lotion or by diamond dermabrasion.
2. Clean the treatment probe by Alcohol, if possible put the probes into the UV sterilizer for 15 minutes.
3. Put the cosmetics product into the bottle of the treatment probe before applying the electroporation treatment.

16. How to maintenance
1. Please clean the treatment probe and the Anode plate after treatment.
2. Plug out the power code from the power socket.
3. Keep the machine in a dry and clean place.